Based on the results of numerical modeling we have analyzed the possibility and calculated the errors of estimating turbulence kinetic energy dissipation rate from data of pulsed Doppler lidar. Compared are the accuracies of estimation of wind velocity spatial structure function from Doppler lidar data obtained by the method of correlation function argument and the method of maximum likelihood. It is shown that, when using sufficiently large number of sample data, the relative error in estimation of turbulence energy dissipation rate with a pulsed Doppler lidar must be below 15–20% at signal-to-noise ratio equal or greater than unity.