The fluorescence spectra of the Rhodamine 6G solution were analyzed. Significant difference was revealed between the spectra recorded in a cell and in droplets of millimeter and micron size. The maximum of the fluorescence band recorded in a droplet shifts to shorter waves by 9 nm with respect to the spectrum recorded in the cell at the power density of laser pumping of 310 MW/cm2. Besides, the relative intensity of the long-wave wing of the fluorescence spectrum decreases. Similar shift of the fluorescence maximum was also detected during the exposure of a jet of micron-size droplets. In the cell containing dye solution, during the exposure in the same range of the pump power density, the shift of the fluorescence spectral maximum was markedly smaller (4 nm).