A system for investigations of the resonance laser ablation effect in polymers and biological tissues was designed, constructed, tested, and evaluated. The laser facility is based on a Sr-vapor laser operating at λ = 6.456, 3.0665, 3.0111, 2.92, 1.0917, and 1.0330 m. The system is capable of measuring laser output parameters varied in a wide range: average power between 1 and 10 W, pulse repetition rate between 1 and 20 kHz, pulse energy between 0.1 and 1 mJ, and energy density between 1 and 20 J/cm2. Experimental data on the resonance laser ablation of polyamide-6 (caprolon) and muscular tissue (bovine muscle) are presented.