The technique has been proposed for calculating the concentration of main constituents of impurities contained in the sea water, which does not require a priori information about the sea water type. It is shown that when variations of main parameters of sea water absorption model and of input parameters are no more than 10%, the error in estimation of the chlorophyll concentration will be no more than 20–25%. The results obtained using this technique have been compared with the direct measurements of chlorophyll concentration.